Nettle (root, stalk and leaves) samples were analyzed by using high- performance liquid chromatography with diode-array detection (hplc-dad) to qualitative and quantitative determination of the phenolic compounds total phenolic components were measured by using folin-ciocalteu method. Reported total dietary intake of phenolics to be approx- imately 1 g/day, regardless of the methods used (hplc or the folin-ciocalteu method) for the determination of the concentration8 thus the quantitative analysis of beneficial com- ponents, such as those responsible for the antioxidative capacity is of great interest. The high contents of total phenolic compounds (23959–24416 mg gae/g of dry phenolic content total flavonoids hplc determination temperature: 25 °c detection: diode-array detector (dad), 205 nm the quantitative determination of chlorogenic acid, apigenin-7-o-glucoside and umbelliferone. The phenolic acids are present in plants and form a diverse group that includes the widely distributed hydroxybenzoic and hydroxycinnamic acids the quantitative analysis of phenolic acids and flavonoids by measurement of ultra- violet absorption is well known (3) other methods for their determination include hplc, gas. To our knowledge, there are no previous reports concerning in vitro antioxidant activities of these plant part extracts the purposes of this study were to determine the total phenolic and the total flavonoid contents, to evaluate their antioxidant activities using 2, 2'-azino-bis (3-ethylbenzothiazoline)-6-sulfonic. Preliminary phytochemical screening, quantitative estimation of total flavonoids, total phenols and antioxidant activity of ephedra alata decne nidal jaradat 1 , fatima hussen 1 and anas al ali 2 1department of pharmacy, faculty of medicine and health sciences, an-najah national university nablus, state of.
Abstract: ageratum conyzoides an annual herbaceous plant is found in tropical and sub-tropical regions present study was aimed to quantitative analysis of total phenolic, total flavonoids, tannin content present in different extracts total phenolic content is significantly high (p005) in n-hexane extract 3462 ± 094 mg of. Total phenolic content was found to be 35725 ± 02336 mg of gae/ 100 g ( aqueous extract), 29616 total flavonoid content was found to be 961556 ± 42664 mg of quercetin equivalent/ 100 g (aqueous extract) quantitative estimation of polyherbal aqueous, hydroalcohol and ethanol extract also. Absorbance/min/g fresh weight of tissue abstract: quantitative estimation of phenolics and related enzymes in insect induced galls (leaf, stem and flower) of salvadora persica were studied the contents of total phenols, o- dihydroxy phenol, peroxidase and polyphenol oxidase activities in galls and normal counter parts of. Of numerous thymus taxa [11-17] using a different approach, this paper reports the qualitative and quantitative analysis of phenolics in different parts of thymus sipyleus for the first time, together with antioxidant activity and total phenolic content experimental chemicals chromatographic grade double-distilled water.
Analysis of total phenolic and total flavonoid contents from b retusa and c javanicum only a few reports described the antioxidant activity and the phenolic content of the stem bark extract of b retusa [8, 9] this is the first report on antioxidant and anticancer activities, and the quantitative estimation of total flavonoid and. Quantitation of total phenolics and flavanoids was done by spectrophotometrically while qualitative analysis was perfomed by paper chromatography and by phytochemical tests our results have shown higher phenolics and flavanoid content in moringa leaf and flower however, higher phenolic content was absent in. Gallic acid was isolated from ethylacetate soluble portion of the methanol extract of fruit pulp of terminalia chebula and characterized by comparing the melting point, rf values, uv and ir spectra with authentic gallic acid quantitative determination of phenolics from different extracts of medicinal herbs such as adhatoda. Suchita g (2017) quantitative estimation of total phenolics and flavonoids in soymida febrifuga leaves am j phytomed clin ther vol 5 no 3:20 introduction soymida febrifuga a juss is a tall tree leaves 23-45 cm long, crowded towards the ends of the branches leaflets 3-6 pairs, opposite, 5-113 by.
This study quantitatively determined the total phenolic contents in ethanolic and aqueous extracts of gul-e-zoofa (flowers of nepeta bracteata benth) using a spectrophotometric method we also performed a spectral study (uv and ir) of the ethanolic extract and a fluorescence study of the powdered drug and successive. Determination of antioxidant capacity, flavonoids, and total phenolic content in eucalyptus and clover honeys maría c kaškonienė v, maruška a, kornyšova o, charczun n, ligor m, buszewski b (2009) quantitative and qualitative determination of phenolic compounds in honey chemical. Quantitative analysis folin ciocalteu (fc reagent) method: (phenolic compounds determination) for the assay, aliquots (100µl) of extracts of different concentrations (10, 20, 30, 40 and 50 µg/ml) were taken in test total flavonoid content was expressed as mg of querectin (que) per gram of dry weight of the extract. The aim of the current study was to evaluate the total phenolic, flavonoid content and to investigate the antioxidant capacities of the fruit extracts of arbutus unedo l that grows in tiaret area (western algeria) first we have extracted the fruit by some non-polar solvent (chloroform, ethyl acetate, 1- butanol) total phenolic.
The quantitative determination of phenolic acids and antimicrobial activity of keywords: symphoricarpos albus (l) blake ( caprifoliaceae), snowberry, phenolic acids, quantitative determination, spectrophotometric each sample was filled up with water to a total of 10 ml the mixture of the above. Quantitative analysis of total phenolic contents and antioxidant activity of fresh field natural rubber latex suwimon siriwong,1 adisai rungvichaniwat,1 pairote klinpituksa,1 khalid hamid musa,2 aminah abdullah2 summary: dried films of fresh field natural rubber latex from pattani local rubber plantation, thailand. The generally accepted reaction for the determination of total phenolic compounds is the folin-ciocalteu assay all of these methods however are inconvenient, stepwise and time-consuming recently, flow injection analysis ( fia) was chosen as an alternative quantitative analysis fia was established in recent decades. The total phenolic and flavonoid content, color, and antioxidant activity were evaluated from ten honey samples from apis mellifera quantitative and qualitative determination of phenolic compounds in honey cheminė technologija, 52(3), 74-80 kishore, r k, halim, a s, syazana, m s n, & sirajudeen, k s n.
The content of biologically active phenolic compounds (total polyphenols, tannins , flavonoids, and phenolic acids) were determined using spectrophotometry in four wild croatian species of daphnel. 632% phenolic acids, 270–552% total phenolic compound content, 572–1 151% keywords: mentha piperita l, lamiaceae, flavonoids, phenolic acids, quantitative analysis, firordt method, dual- wavelength spectrophotometer peppermint (mentha piperita l, lamiaceae) is a medicinal plant that is widely used in. Phenolic compounds was carried out in triplicate the total phenolic content was expressed as milligrams of gallic acid equivalents (gae) per g of dried sample colorimetric estimation of total flavonoid content11 aluminium chloride colorimetric technique was used for flavonoids estimation 10 mg of rutin.
Leaves and h halimifolium flowers respectively the extracts were suspended in meoh: h2o (3: 7 v/v) and extracted successively with dichloromethane, n- butanol, ethyl acetate in this order determination of total antioxydant compounds total polyphenol content the total phenolic content. Quantitative analysis of total phenolic content in avocado ( persia americana) seeds in eastern province of kenya p githinji1 , l gitu1, e marete2, m githua2, m mugo2, and e n mbaka3 1jomo kenyatta university of agriculture and technology, nairobi, kenya 2meru university of. Flavonoids and other phenolics for the medicinal property, especially their potential role in the prevention of cancer and heart diseases9this study presents quantitative estimation of total flavonoids and total phenolics contents from the fruits of tterrestris by spectrophotometric method experimental: plant material was. Quantitative estimation of total phenols in calyptocarpus vialis - an emerging weed in karnataka kavitha sagar botanical garden & herbarium, uas, gkvk, bengaluru, karnataka 560 065 received: 24 september 2016 revised: 22 october 2016 key words: aggressive, calyptocarpus vialis, karnataka, phytochemical,.
Quantitative determination of total phenols was done on the basis of a standard curve of gallic acid and linearity of the calibration curve was achieved between 625 to 100 mg/ml concentration for gallic acid (r2=09926 fig 1) quantitative determination of total flavonoids was done on the basis of a standard curve of rutin.